==================================CMR34================================== 34. This search is to be conducted on the clinical use of electrical equipment, to any part of the body, including ECT, for the treatment of carcinoma and sarcoma. 1 UI - 87088815 AU - Herzog R ; M:uller-Wellensiek A ; Voelter W TI - Usefulness of Ficoll in electric field-mediated cell fusion. AB - Electrofusion is a technique that enables the production of new cell types with desired properties to be done. Ehrlich ascites tumor cells are fused by means of an electric field. Under too harsh external field strength or pulse length conditions, however, membrane breakdown leads to a loss of cellular cytoplasm. Addition of the high polymer Ficoll to the fusion medium increases its density and osmotic pressure, thus preventing cytoplasm from running out and, therefore, maintaining cell viability. Fusion between cells of different sizes is made possible or facilitated by means of Ficoll, as big cells do not lose their cytoplasm under conditions required for enhancing membrane permeability of the small cells. In presence of the proteolytic enzyme pronase, addition of Ficoll to the fusion medium further raises the fusion percentage compared to the exclusive addition of pronase. The fusion of cells of different densities is also greatly facilitated and its percentage increased by addition of Ficoll, thus obviating the necessity to perform electrofusion under conditions of microgravity. MH - Animal ; Carcinoma, Ehrlich Tumor/ENZYMOLOGY ; *Cell Fusion ; DNA/ ANALYSIS ; Electricity ; Female ; *Ficoll ; Lactate Dehydrogenase Isoenzymes/ANALYSIS ; Mice ; Microscopy, Phase Contrast ; *Polysaccharides SO - Life Sci 1986 Dec 15;39(24):2279-88 2 UI - 87065275 AU - Setka J ; Mandys V ; Vrana J ; Hole:ckov:a E TI - Relation between high-frequency electrical impedance and malignity of two L cell sublines. AB - Two L cell sublines (L-As and LC3), differing in the ability to induce sarcomas in irradiated C3H mice, were grown on special gold electrodes interrupted in the middle by a 50 micron wide slot. Electrical impedance of the two sublines was compared at 100 kHz in a special measuring device connected with an apparatus used for impedance measurement in clinics. Significantly lower values of impedance characterized the LC3 cells, which induced more sarcomas in susceptibility hosts than the L-As cells. This result agrees with the findings in human tumors compared with corresponding normal tissue or benign growth, and stresses the importance of the cell population as such in impedance measurements. MH - Animal ; Cell Line ; *Electric Conductivity ; Female ; L Cells/ *PHYSIOLOGY ; Mice ; Mice, Inbred C3H ; Sarcoma, Experimental/ *PHYSIOPATHOLOGY SO - Neoplasma 1986;33(5):609-13 3 UI - 86213731 AU - Giam YC ; Ong BH TI - Bowenoid papulosis: a recent entity in sexually transmitted diseases. AB - Bowenoid papulosis is a recent phenomenon of multiple lesions with a histology of carcinoma in situ appearing on the genitals of young men. We report 6 male patients with this condition presenting with warty and/or lichen planus like lesions on the genitals. The histology shows carcinoma in situ. Three (50%) showed spontaneous regression, and the rest had persistence and recurrence. Repeat biopsy of one recurrent lesion showed a verruca. The evidence for a possible viral aetiology will be discussed. Long term studies are required to assess any oncogenic potential. As it is thought to be presently a viral infection with epidermal atypia, effective conservative management is sufficient. MH - Adult ; Carcinoma in Situ/PATHOLOGY/SURGERY ; Case Report ; Electrocoagulation ; Human ; Male ; Neoplasm Regression, Spontaneous ; Penile Diseases/*PATHOLOGY/SURGERY ; Penile Neoplasms/PATHOLOGY/SURGERY ; Sexually Transmitted Diseases/ *PATHOLOGY/SURGERY ; Warts/*PATHOLOGY/SURGERY SO - Ann Acad Med Singapore 1986 Jan;15(1):15-9 4 UI - 86320539 AU - Lazarus SC ; McCabe LJ ; Nadel JA ; Gold WM ; Leikauf GD TI - Effects of mast cell-derived mediators on epithelial cells in canine trachea. AB - We examined the interaction between mast cell-derived mediators and the electrical and ion transport properties of canine tracheal epithelium. We compared the effect of mediators released by immunologic challenge of sensitized lung parenchyma with that of mediators released from canine mastocytoma cells challenged with calcium ionophore A23187. Short-circuit current (Isc) increased by 19.2 +/- 3.0 microA/cm2 in response to mediators released from sensitized lung fragments challenged with ragweed antigen. This effect was not due to histamine. When the epithelial tissues were pretreated with indomethacin, the same mediator supernatant increased Isc by only 3.8 +/- 4.3 microA/cm2. The mediators released from 10(7) mastocytoma cells challenged with calcium ionophore increased Isc by 25.1 +/- 13.6 microA/cm2. In the presence of indomethacin, the Isc increased by 2.0 +/- 0.4 microA/cm2. Mastocytoma-derived mediators produced an increase in net chloride secretion without a significant effect on net sodium absorption. This study provides direct evidence that mast cell-derived mediators can stimulate epithelial ion transport in canine trachea and suggests that the effect is indirect and dependent on intact cyclooxygenase pathways in the tracheal epithelium. MH - A-23187/PHARMACODYNAMICS ; Animal ; Biological Transport/DRUG EFFECTS ; Dogs ; Electric Conductivity ; Electrophysiology ; Epithelium/PHYSIOLOGY ; Indomethacin/PHARMACODYNAMICS ; Ions ; Lung/IMMUNOLOGY ; Mast Cells/*PHYSIOLOGY ; Mice ; Mice, Nude ; Pollen/IMMUNOLOGY ; Prostaglandin Synthase/PHYSIOLOGY ; Sarcoma, Mast Cell/PHYSIOPATHOLOGY ; Support, Non-U.S. Gov't ; Support, U.S. Gov't, P.H.S. ; Trachea/*PHYSIOLOGY SO - Am J Physiol 1986 Sep;251(3 Pt 1):C387-94 5 UI - 86314263 AU - Turney TH ; Harmsen AG ; Jarpe MA TI - Modification of the antitumor action of Corynebacterium parvum by stress. AB - Social grouping and isolation of mice, in the presence of an acute stressor, were found to differentially affect the antitumor action of the immunological adjuvant Corynebacterium parvum. Socially grouped DBA/2j mice were injected intradermally with P815 mastocytoma ascites cells. Half the mice had a threshold dose of C. parvum admixed with the P815 cells. Half the mice in each of those conditions were given acute, inescapable electric footshock. In a second experiment, the stressed mice were socially isolated prior to the acute stress. Tumor growth itself was not affected by the stress procedures. C. parvum inhibited tumor growth in non-stressed and socially isolated, stressed mice. However, social grouping selectively negated the C. parvum effect resulting in tumor growth and mortality equivalent to mice not given the adjuvant. Psychological factors may be important to the development of concomitant immunity and the efficacy of immunotherapies. MH - Adjuvants, Immunologic/*THERAPEUTIC USE ; Animal ; Electroshock ; Male ; Mice ; Mice, Inbred DBA ; Neoplasm Transplantation ; Neoplasms, Experimental/COMPLICATIONS/*THERAPY ; Propionibacterium acnes/*IMMUNOLOGY ; Sarcoma, Mast Cell/THERAPY ; Social Isolation ; Stress, Psychological/*COMPLICATIONS SO - Physiol Behav 1986;37(4):555-8 6 UI - 86285940 AU - Lenehan PM ; Meffe F ; Lickrish GM TI - Vaginal intraepithelial neoplasia: biologic aspects and management. AB - A review of 59 consecutive cases of vaginal intraepithelial neoplasia seen in a colposcopy clinic between 1971 and 1984 was made. All patients presented with an abnormal Papanicolaou smear, and diagnosis was confirmed by colposcopically directed biopsy. The patients ranged in age from 23 to 72 years, with a mean age of 49. Fourteen (24%) patients were less than 40 years of age. A previous hysterectomy had been performed in 68% of patients, 42.5% of these being for benign disease. Forty-two patients (71%) had evidence of multicentric neoplasia of the lower genital tract, and 30 (51%) had been treated previously for cervical neoplasia. Twenty-nine lesions were multifocal and 52 (88%) were located in the upper third of the vagina. The results of the different modes of treatment were reviewed. Surgical extirpation gave the best results. The local ablative techniques used, ie, electrocautery and CO2 laser, were associated with failure rates of 25 and 50%, respectively. Three patients developed invasive carcinoma of the vagina after treatment. MH - Adult ; Aged ; *Carcinoma in Situ/DIAGNOSIS/SURGERY ; Cervix Neoplasms/SURGERY ; Electrosurgery ; Female ; Human ; Lasers/ THERAPEUTIC USE ; Middle Age ; Neoplasm Recurrence, Local/SURGERY ; Neoplasms, Multiple Primary/DIAGNOSIS ; Support, Non-U.S. Gov't ; Time Factors ; *Vaginal Neoplasms/DIAGNOSIS/SURGERY SO - Obstet Gynecol 1986 Sep;68(3):333-7 7 UI - 86208429 AU - Ferguson EF Jr ; Anderson AW 4th TI - Leiomyosarcoma of the rectum. AB - We have reported a case of leiomyosarcoma of the rectum. These tumors comprise 0.1% of all rectal tumors, with about 100 cases having been reported. Symptomatology is nonspecific; digital examination is the most effective method of detection. Local surgical excision is as effective as abdominoperineal resection. MH - Case Report ; Electrocoagulation ; Female ; Human ; Leiomyosarcoma/*DIAGNOSIS/SURGERY ; Middle Age ; Rectal Neoplasms/ *DIAGNOSIS/SURGERY SO - South Med J 1986 May;79(5):645 8 UI - 86188917 AU - Baskin DS ; Mehler WR ; Hosobuchi Y ; Richardson DE ; Adams JE ; Flitter MA TI - Autopsy analysis of the safety, efficacy and cartography of electrical stimulation of the central gray in humans. AB - Electrical brain stimulation is effective in controlling certain intractable chronic pain syndromes in humans, but the specific target site(s) for stimulation producing a maximal analgesic effect is (are) not well defined. This prospective study correlates the clinical results of chronic stimulation of the periaqueductal gray (PAG) and periventricular gray (PVG) matter in humans with the anatomic site of electrode placement as determined at autopsy, and documents the histologic reactions to electrode implantation and electrical stimulation of the area. Seven patients underwent electrode implantation to control their chronic pain; two had electrodes implanted bilaterally. All patients obtained complete analgesia with stimulation, although 3 subsequently found the stimulation to have diminished efficacy. The opiate antagonist naloxone reversed the analgesia in the 4 patients so tested. All 7 patients later died of causes unrelated to electrode implantation or stimulation. Postmortem analysis showed that, for 6 of the 9 electrodes implanted, the electrode tip was located in the ventrolateral PAG at the level of the posterior commissure; the other 3 electrodes were found in the white matter adjacent to the PAG. No evidence of gliosis or parenchymal reaction was observed along the tracts and tips of the electrodes. The results indicate that the ventrolateral PAG and PVG matter at the level of the posterior commissure is the optimal site for therapeutic electrical brain stimulation for opiate-responsive pain in humans. MH - Adult ; *Brain Mapping ; Carcinoma/*PATHOLOGY/THERAPY ; Case Report ; Diabetic Neuropathies/*PATHOLOGY/THERAPY ; Electric Stimulation/ADVERSE EFFECTS/INSTRUMENTATION/*METHODS ; Electrodes, Implanted ; Electrotherapy/ADVERSE EFFECTS/ INSTRUMENTATION/METHODS ; Female ; Human ; Male ; Middle Age ; Periaqueductal Gray/*PHYSIOPATHOLOGY ; Prospective Studies ; Time Factors SO - Brain Res 1986 Apr 23;371(2):231-6 9 UI - 86172045 AU - Streem SB ; Pontes EJ TI - Percutaneous management of upper tract transitional cell carcinoma. AB - Conservative (nephron-sparing) surgery for transitional cell carcinoma of the upper tract generally has entailed variations of partial nephrectomy or open pyelotomy with excision and fulguration of tumor. We report on a patient in whom percutaneous technology was used successfully to manage transitional cell carcinoma of the lower infundibulum after other treatment options had been exhausted. MH - Aged ; Carcinoma, Transitional Cell/DRUG THERAPY/*SURGERY ; Case Report ; *Electrosurgery ; Human ; Kidney Neoplasms/DRUG THERAPY/ *SURGERY ; Male ; Mitomycins/THERAPEUTIC USE ; *Nephrostomy, Percutaneous SO - J Urol 1986 Apr;135(4):773-5 10 UI - 86152250 AU - Himal HS TI - Therapeutic endoscopy. AB - The development of flexible endoscopic instruments has altered the surgical approach to many disease processes. Endoscopic treatment of gastrointestinal polyps, common bile duct stones and foreign bodies of the gastrointestinal tract has now become the preferred method of treatment. Endoscopic gastrostomy is replacing surgical gastrostomy for the patient who requires long term enteral nutrition. Sclerosis of esophageal varices has now become the preferred method of managing patients with portal hypertension and bleeding esophageal varices. As newer endoscopic instruments are developed, more and more operative procedures will be carried out by endoscopic methods. MH - Biliary Tract Neoplasms/SURGERY ; Carcinoma/SURGERY ; Colonic Diseases/SURGERY ; Colonoscopy/METHODS ; Electrocoagulation ; *Endoscopy/METHODS ; Esophageal Neoplasms/SURGERY ; Esophageal Stenosis/SURGERY ; Fiber Optics ; Foreign Bodies/SURGERY ; Gastrointestinal Diseases/*SURGERY ; Hemorrhage, Gastrointestinal/ THERAPY ; Human ; Intestinal Polyps/SURGERY ; Intubation, Gastrointestinal ; Lasers/THERAPEUTIC USE ; Pancreatitis/SURGERY ; Review ; Sclerosing Solutions/THERAPEUTIC USE ; Vater's Ampulla/ SURGERY SO - Surg Gynecol Obstet 1986 Mar;162(3):291-9 11 UI - 86127521 AU - Deigan EA ; Carmichael JA ; Ohlke ID ; Karchmar J TI - Treatment of cervical intraepithelial neoplasia with electrocautery: a report of 776 cases. AB - From 1973 to 1984, 776 patients with cervical intraepithelial neoplasia were treated with outpatient electrocautery (hot cautery) without anesthesia. Of these, 726 (94%) were available for follow-up in 3 to 6 months. An initial cure rate with one treatment of 89% to 90% was achieved. Cure rates were similar for all degrees of dysplasia, including carcinoma in situ. There were no complications. All patients with failure of the initial treatment who returned for further outpatient management were eventually cured with use of electrocautery. Long-term follow-up rates ranged from 75% at 1 year to 46% at 5 years. There were few late recurrences, most of which were treated again (successfully) with electrocautery. Electrocautery produces cure rates similar to those for other forms of conservative management and may be the most cost-effective method of management of cervical intraepithelial neoplasia. MH - Ambulatory Surgery ; Carcinoma in Situ/*SURGERY ; Cervix Dysplasia/*SURGERY ; Cervix Neoplasms/*SURGERY ; Colposcopy ; Comparative Study ; *Electrocoagulation ; Female ; Follow-Up Studies ; Human ; Neoplasm Recurrence, Local/*OCCURRENCE ; Ontario ; Outpatient Clinics, Hospital ; Support, Non-U.S. Gov't ; Time Factors SO - Am J Obstet Gynecol 1986 Feb;154(2):255-9